|
KMID : 1039120160050020175
|
|
Clinical and Experimental Vaccine Research
2016 Volume.5 No. 2 p.175 ~ p.178
|
|
|
A Bordetella pertussis proteoliposome induces protection in mice without affecting the immunogenicity of diphtheria and tetanus toxoids in a trivalent formulation
|
|
Castillo Sonsire Fernandez
Chovel Mario Landys
Hernandez Niurka Gutierrez
Gonzalez Lorena Corcho
Blanco Amaya
Hernandez Daily Serrano
Tito Maydelis Alvarez
Perez Jose Luis
|
|
|
Abstract
|
|
|
|
In this study, a formulation of Bordetella pertussis proteoliposome (PLBp), diphtheria, and tetanus toxoids and alum (DT-PLBp) was evaluated as a trivalent vaccine candidate in BALB/c mice. Vaccine-induced protection was estimated using the intranasal challenge for pertussis and enzyme-linked immunosorbent assay fvto assess serological responses for diphtheria or tetanus. Both, diphtheria-tetanus-whole cell pertussis (DTP) and diphtheria-tetanus vaccines (DT) were used as controls. Animals immunized with DT-PLBp, PLBp alone, and DTP showed total reduction of CFU in lungs 7 days after intranasal challenge. Likewise, formulations DT-PLBp, DTP, and DT elicited antibody levels ≥2 IU/mL against tetanus and diphtheria, considered protective when neutralization tests are used. Overall, results showed that combination of PLBp with tetanus and diphtheria toxoids did not affect the immunogenicity of each antigen alone.
|
|
|
KEYWORD
|
|
|
Bordetella pertussis, Proteoliposome, Whooping cough, Vaccines
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
  
|